Can this be removed by PCR cleanup or is there any PCR cleanup protocol to enhance the result?3mo ⋅ evi1ang1e ⋅ r/molecularbiology
I'm in med school, 1st year. This my molecular biology lecture outline for the semester. Could anyone help suggest what would be the most high yield resource(book or videos) i could use, especially for the PCR tests10mo ⋅ songflames ⋅ r/molecularbiology
I need helping solving this restriction mapping. No clue where to start 😪1yr ⋅ No_You_tell_me ⋅ r/molecularbiology
This is a question from my textbook. I'm confident that d is the correct option, but my classmate insists that e is the correct option. If that is the case, I struggle to understand how a inducible promoter is essential characteristic of an expression vector. Any help is appreciated!1mo ⋅ Civil-Fudge6585 ⋅ r/molecularbiology
Why is this RNA oligo making a weird donut shape in my urea gel?2yr ⋅ academic_wackademic ⋅ r/molecularbiology
can someone explain to me what an open reading frame is? and whats the difference between an ORF and a reading frame?2yr ⋅ P0L4RST4R ⋅ r/molecularbiology
I did EMSA using glutamate buffer (composition: 600mM HEPES and 12.5mM potassium glutamate). But when I scanned it, I saw bands running the same size in every lane (even without any protein!) Does anyone know what this is? Which buffer do you use usually for EMSA?1yr ⋅ Xenos_in_science ⋅ r/molecularbiology
help with agarose gel electrophoresis reading. This is my first agarose gel electrophoresis on pcr. First lane is DNA ladder, 2nd and 3rd lane are from the same DNA sample. Is the resolution good enough? And what can you deduce with the DNA ladder?1yr ⋅ lilianredditor ⋅ r/molecularbiology
What is that black dot thing on my Western blot membrane?1yr ⋅ Mammoth_Housing_4420 ⋅ r/molecularbiology
Alberts Molecular Biology of the Cell 7th edition - international student edition?1yr ⋅ Ok_Level2393 ⋅ r/molecularbiology
Does anything scream red flag about my friend’s PCR? I’m trying desperately to troubleshoot.. no bands9mo ⋅ bluish1997 ⋅ r/molecularbiology
Yeah... I broke the pipette's tip :') has anyone ever experienced this? If so, how did you fix it?2yr ⋅ CellanKnight ⋅ r/molecularbiology
Hi, so here I am supposed to compare the samples (A B C) to the Marker and to determine how many basepairs that is an the intensiti and also concentration in ng/mikrol. I am sorry if this sounds dumb but english isnt my first language. All help is appreciated6mo ⋅ braankec ⋅ r/molecularbiology
Sorry, this is a repost from a question I asked in another subreddit Blurry Agarose Gel Electrophoresis From left to right: DNA Ladder, digested plasmid, undigested plasmid, genomic DNA Anyone know why my gel would come out looking like this? 25mL, 0.7% Agarose, made with 0.5X TBE Buffer7yr ⋅ Tabber42 ⋅ r/molecularbiology
